apasin过表达对Tca8113细胞增殖和凋亡的影响

Purpose: To construct the human tapasin expression vector and to evaluate its efects on the proliferation and apoptosis of oral cancer Tca8113 cells in vitro. Methods and Materials: Full sequence of tapasin cDNA was amplified from normal oral mucosa tissue samples by RT-PCR and cloned into pCMV-Tag3B vector. Western blot was performed to detect expression levels of tapasin in Tca8113 cells. The cell proliferation was measured by MTT, the distribution of the cell cycle and apoptosis were detected by flow cytometry.Results: Tapasin cDNA over-expression vector of pCMV-Tag3B-tapasin was successfully constructed. The expressions of tapasin in Tca8113 cells，including protein level was significantly increased after stable transfection, which could inhibit the proliferation of the oral cancer cell line Tca8113 significantly and induce its apoptosis. FACS analysis showed a decrease in S population and a significant increase in G1 population after transfection (P<0.01). Conclusion: The over-expression of tapasin can inhibit cell proliferation, decreases S population and increases G1 population and induce cell apoptosis. Therefore，tapasin will be considered as a target gene of cancer gene therapy.