苎麻叶片再生及根癌农杆菌介导的遗传转化参数研究
Plant regeneration from ramie leaves and primary study on factors affecting Agrobacterium-mediated transformation
以苎麻试管苗叶片为外植体进行组织培养和遗传转化，能保持母本的生物学特性。本研究以"华苎4号"和"华苎5号"试管苗叶片为外植体，研究了不同培养基配方对再生的影响，使得再生频率达到或者接近50%。"华苎4号"试管苗叶片最适再生培养基为MSB + 0.5 mg/L thidiazuron（TDZ） + 0.01 mg/L IAA，"华苎5号" 试管苗叶片最适再生培养基为MS + 0.5 mg/L TDZ + 0.01 mg/L NAA。在根癌农杆菌介导的苎麻叶片遗传转化中，当共培养时间为72 h、乙酰丁香酮（AS）浓度在50 ~ 100 mg/L、菌液浓度在OD6000.5 ~ 0.8 时能够得到较高的遗传转化效率，为根癌农杆菌介导的苎麻遗传转化提供参考。
Using leaves derived from test-tube plantlets as explants in ramie tissue culture and Agrobacterium-mediated transformation can maintain the biological characters of the original plants. Leaves explants derived from test-tube plantlets of Huazhu No. 4 and Huazhu No. 5 were used to evaluate the effects of different media on plant regeneration in this study, in which the highest regeneration ratio was up to 50%. The optimum medium for plant regeneration from Huazhu No. 4 was MSB medium supplemented with 0.5 mg/L thidiazuron (TDZ) and 0.01 mg/L IAA, and the best one for Huazhu No. 5 was MS medium containing 0.5 mg/L TDZ and 0.01 mg/L NAA. In the Agrobacterium-mediated transformation of ramie, the optimal cocultivation duration, acetosyringone (AS) concentration and Agrobacterium concentration was 72h, 50-100 mg/L and OD6000.5- 0.8, respectively, which will be a practical tool for the ramie transformation in the near future.
杨金雨、王绪霞、蒋卉、张金果、彭定祥、刘立军、刘刚、汪波
生物工程学植物学遗传学
作物遗传育种学转基因GFP植株再生
rop genetics and breedingtransformationGFPplant regeneration
杨金雨,王绪霞,蒋卉,张金果,彭定祥,刘立军,刘刚,汪波.苎麻叶片再生及根癌农杆菌介导的遗传转化参数研究[EB/OL].(2011-02-18)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201102-291.点此复制
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