原花色素对口腔细菌及牙菌斑生物膜影响的实验研究

Objective To evaluate the effect of PA on the oral bacteria and on the biofilm formation and metabolism Methods The minimum inhibitory concentrations (MIC) were determined using the microdilution method. The effect of PA on the acid production of oral bacteria were evaluated at a sub-MIC concentration. The biofilm formation and metabolism were determined by crystal violet stain and XTT method. Results ① PA inhibited the growth of tested oral bacteria. The M IC of PA on S. mutans and S.gordonii was 4m g /m l , on F.nucleatum and P.gingivalis was 1mg/ml and 0.25mg/ml ; ② PA can inhibit the acid production of S. mutans signifigently ； ③ it was also proved that the growth and the metabolism of biofilm formed by S. mu tans, would be inhibited under the effect of PA .Conclusion Proanthocyanidin effectively infected the cariogenicity of oral bacteria and biofilm, Which may be a promising natural agent for caries therapy.