热致形状记忆性人工晶状体材料的研究

Objective] Study the effects of different molecular weights and concentrations of the crosslinking agents on the new thermally induced shape-memory intraocular lens materials. [Methods] Use bending test to detect the shape-memory properties in simulated vivo condition. Use contact angle measuring instrument to detect the infiltration. Measure the cytotoxicity with CCK-8 assay. Use the crystal violet assay to detect lens epithelial cell adhesion on the material surface. Observe the surface protein adsorption under fluorescent microscope after 2 hours cultured with fluorescently labeled fibronectin. Research the lens epithelial cell migration below the material with cell culture chamber. [Results] Eight test materials can fully restore to its original shape within 40 seconds, and show no significant cytotoxicity. The materials of crosslinking molecular weight 400, with more LEC adhesion, fibronectin adsorption and higher adhesion characteristics, LEC migration below the material are blocked. However, the materials of crosslinker molecular weight 1000, with the crosslinking agent concentration increases, the adhesion characteristics decrease and LEC migration increase. Therefore, the materials of crosslinking molecular weight 400 show better capsular biocompatibility.