SLP-2基因在乳腺癌细胞系中的表达及对MCF-7细胞增殖的影响

Breast cancer is locally advanced at the time of diagnosis,and its occurrence, evolution, invasion and metastasis is a gradual accumulation process of multi-genes participating, it also involves gene regulation, expression, mutation and many other changes. Human stomatin like protein 2 (SLP-2) is a novel cancer-related gene,it played important roles in a variety of tumors, especially high-level SLP-2 expression were associated with significantly decreased survival in breast cancer.To study the function of SLP-2 in breast cancer furtherly,we detected the expression of SLP-2 protein in different breast cancer cells by Western blotting,and found that the expression of its protein in breast cancer cells increased with the increase of the degree of cancer.We also successfully constructed recombinant plasmid pCMV-Myc-SLP-2 and demonstrated it can expressed well in MCF-7. The MTT assay showed that the cell proliferation was significantly inhibited after transfection of pCMV-Myc-SLP-2, we also found that the expression of SLP-2 and HER2/nue have negative correlation in MCF-7, thus providing a useful clue for further research on the occurring and development of breast cancer.