温度、水杨酸对葡萄果实二氢黄酮醇还原酶（DFR）的影响

Effects of low temperature (8℃) acclimation and high temperature (45℃) stress on dihydroflavonol 4-reductase (DFR) were investigated in grape berries （Vitis vinifera L. cv. Cabernet Sauvignon）and the impact of salicylic acid (SA) on the gene expression of DFR at enzymic activity, translational and transcriptional levels was also analyzed. The results showed that the expression of the gene encoding DFR increased at mRNA, protein and activity levels, respectively, at the early period of low temperature acclimation. The gene expression at various levels was present more significant increase in the low temperature-acclimated grape berries during heat stress than that in the non-low temperature acclimated berries, which indicated that low temperature acclimation could promote the adaption of grape berries to heat stress. SA pretreatment also activated the gene expression of DFR at various levels. It was thus suggested that both temperature acclimation and SA were involved in the regulation of DFR gene expression, and the regulation was rapid and sensitive.