人巨细胞病毒在Rasmussen脑炎中的表达分析
nalysis of Protein and Nucleic Levels of the Human Cytomegalovirus in Rasmussen Encephalitis
目的：探究人巨细胞病毒（HCMV）与Rasmussen脑炎（Rasmussen's Encephalitis, RE）发生发展的相关性。方法：利用免疫组化染色方法，检测RE患者手术脑组织标本中HCMV特异性蛋白pp65的分布，与对照组相对比，分析HCMV感染或携带与RE发病的相关性。结果：7例RE患者除去1例患者行活检手术外，对全部26例行半球手术的RE患者脑组织的石蜡包埋切片进行免疫组化和原位杂交试验，免疫组化结果显示HCMV-pp65抗原在其中23例中阳性表达，阳性率88.46%，对比正常对照组结果，阳性结果具有统计学意义。结论：RE的发生与HCMV感染具有相关性。HCMV作为诱发因素，参与了RE的发病起始和疾病的病程进展，但没有参与直接攻击中枢神经系统，没有直接导致RE病损区的病理变化。HCMV作用于RE病损区的神经元胞膜和突起，调节宿主细胞的代谢，未直接影响细胞核，未直接改变细胞的理化性质。
Object: To study the relation between human cytomegalovirus (HCMV) infection or carrier and the occurence of Rasmussen's Encephalitis (RE) patients. Method: Brain tissue of RE group and control group were studied by pp65 immumohistochemical staining. By statistic analysis, compared the result of two groups. Result: Form April 2008 to December 2013, The epilepsy center of Beijing Sanbo Brain Hospital performed surgeries on 27 patients with RE (RE group, n = 27), autopsy center of Beijing University offered 6 cases of postmortem brain tissue specimens without epilepsy or nervous system diseases, and no poison death (normal control group, n = 6). Immunohistochemical results showed that positive expressions of HCMV-pp65 antigen in 23 cases, with positive rate 88.46%, which was significantly higher than the control group. Conclusion: HCMV infection is associated with the occurrence and progress of RE as a trigger factor, but no attack CNS and no involved in the pathological changes directly HCMV infections act on the membrane and processes of neurons.
张尧、王一松、栾国明、关宇光、安静
神经病学、精神病学基础医学微生物学
Rasmussen脑炎发病机制免疫组化人巨细胞病毒
Rasmussen's encephalitispathogenesismmunohistochemistryhuman cytomegalovirus (HCMV)
张尧,王一松,栾国明,关宇光,安静.人巨细胞病毒在Rasmussen脑炎中的表达分析[EB/OL].(2015-10-15)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201510-71.点此复制
评论