海洋链霉菌Streptomyces sporoverrucosus 33510次级代谢产物研究

o study active secondary metabolites of marine actinobacteria Streptomyces sporoverrucosus 33510, and evaluate antibacterial activity of the purified compounds. The Streptomyces sporoverrucosus 33510 strain was tested for antibacterial activity. Streptomyces sporoverrucosu 33510 shows strong inhibitory activity against various plant pathogenic bacteria. The compounds were isolated and purified by high performance liquid chromatography (HPLC), semi-pre HPLC, thin-layer chromatography (TLC) and resortification technology. And their chemical structures were characterized by nuclear magnetic resonance (NMR) analysis. The chemical structures were identified as bisphenol A (1), 2-(4-methoxyphenyl)acetic acid (2), N-phenethylacetamide (3), methyl 2-(1H-indol-3-yl)acetate (4), dibutyl phthalate (5), cyclo(D)-pro-(D)-Leu (6), cyclo(D-Pro-L-Leu) (7), cyclo-(D-ProL-Ile) (8), cyclo(L-Pro-L-Phe) (9), cyclo-(L-Leu-L-Val) (10). Except for the compounds 5 and 9, the remaining eight compounds were isolated from Streptomyces sporoverrucosus for the first time. The antibacterial activity of the compounds were tested for by Disk Diffusion Assay. Compound 3, 4, 5, and 6 show antibacterial activity against Rhizoctonia solani. Compound 7, 8, and 9 show antibacterial activity against Cryphonectria parasitica. Compound 3 and 7 show antibacterial activity against Fusarium pseudograminearum. Marine actinomycetes Streptomyces sporoverrucosus 33510 can be a potential resource for antibacterial drugs.