胶质瘤细胞中ADAR2 mRNA前体选择性模式的分析

Objective：To analysis the differences of ADAR2 alternatively splicing pattern between glioma cell lines and normal human astrocyte. Methods：Real-time PCR was performed to detect the expression level of each alternatively splicing variant using specific primer which was confirmed to amplify only the targeted template and not other alternatively spliced variant fragments. Results：Real-time PCR revealed that the ADAR2 pre-mRNA splicing pattern has no significant difference at exon 1a and exon 2 between glioma cell lines and normal human astrocyte; At the same time, we detected that the amount of alternative splicing variants including exon 5a was higher than that of alternative splicing variants not including exon 5a in human glioma cell lines, however, the expression of alternative splicing variants including exon 5a was lower than that of alternative splicing variants not including exon 5a in human astrocyte. Conclusion：The different alternatively splicing pattern at exon 5a may attribute to the decreased activity of ADAR2..