柑橘愈伤组织中线粒体DNA含量增加

Mitochondrial genome (mtDNA) genetic stability was investigated in long-term sub-cultured Citrus callus by restriction fragment length polymorphism (RFLP) and histological observations. The results revealed that mtDNA performed high stabilities in old-cultivated species, and variations were observed among several samples with hybrid-origin. However, mtDNA abundance increased among eighteen out the twenty-three calluses, the ratios ranged from 1.2 to 6.4 times. Dot blotting of the nuclei DNA with mitochondrial specific probes showed that more intensive hybridization signals in callus than those in the corresponding control leaves, that indicated that mtDNA transfer to the nuclei probably be accelerated in callus.