外源GA3对盐环境下锁阳种子萌发的调控

o understand the growth character of halophytic medicinal root parasitic plant Cynomorium songaricum Rupr., we use C. songaricum seeds to study the germination and proteomics in order to reveal the role of salt and GA3 in the establishment of relationship between C. songaricum seed and its host. We added exogenous GA3 in salt environment and recovery conditions, determined the proteomics and the percentages of seed germination, recovery germination and final germination under different temperature conditions. The result showed that exogenous GA3 can increase the germination percentage of C. songaricum seed under NaCl environment and recovery conditions, under the conditions of 25 C, 200μmol/L, GA3+100mmol/L NaCl, the C. songaricum seed reached highest germination percentage 7.00±1.91%, the non-germinated seeds were transferred to another petridish under condition of 25 C, 200 μmol/L GA3 for recovery germination, the germination percentage reached maximum (32.32 ± 2.89%). After 40 d treatment, the final germination percentage were mainly contributed from recovery germination stage, salt environment with GA3 was regarded as the preconditioning stage of seed germination, and it can activate the gibberellin receptor (GID1).The preculture seeds germ tube was longer and could prevent browning, the haustorial was more obvious. So exogenous GA3 can induce and promote C. songaricum seed germination under salt and the salt reduced environment.