N-乙酰半胱氨酸治疗D-氨基半乳糖诱导猪急性肝衰竭对血氨、内毒素和细胞炎症因子的影响研究

Objective To study changes of serum ammonia, endotoxin and inflammatory factor of N-acetylcysteine on D-galactosamine-induced acute hepatic failure in pigs. Methods Sixteen minipigs were divided into two groups at random: normal control group （n=2）, acute failure group（n=6）and treatment group(n=8). The acute failure model of pig was induced by D-galactosamine (dosage, 1.2g/kg). The treatment group was treated with NAC，and the acute failure group was administered with 0.9%NaCl. The blood ammonia, plasma endotoxin and inflammatory cell factor (TNF-α, IL-1β) were tested. Results: Compared with acute failure group, the level of blood ammonia and inflammatory cell factor were significantly decreased at 24 and 48 hours, plasma endotoxin was significantly fall at 12 hours in the treated group: blood ammonia (24 hours：112.5±21.7 vs 95.4±25.9, P<0.05; 48 hours: 257.5±50.7 vs 213.6±70.5, P<0.05), plasma endotoxin(12hours: 0.165±0.05 vs 0.13±0.01, P<0.05), TNF-α (24 hours: 24.0±5.3 vs 19.3±4.2, P<0.05; 48 hours: 29.2±8.7 vs 26.1±3.8, P<0.05), IL-1β(24 hours: 18.5±3.6 vs 15.2±3.4, P<0.05；48hours：24.3±6.3 vs 20.1±5.2，P<0.05). Conclusion: NAC can effectively protect hepatocyte function from injures, ameliorate ammonemia and endotoximia and inhibit Inflammatory reaction.