健骨颗粒对体外破骨细胞整合素αν、β3mRNA表达的影响

o investigate the effects of Jiangu grunula(JGG) of traditional Chinese drug which tonifying the kidney and benefiting the spleen on the expression of integrinαv、β3 in osteoclast in vitro. Methods: The preosteoclasts of RAW264.7 cell were induced to mature osteoclast by RANKL and M-CSF. Then cultured the mature osteoclast and the JGG or normal saline(NS) contained serum were respectively added . The expression of Itgαv、β3 was measured by real time RT-PCR..Then the number and area of bone resorption pits on bone slices stained by methods of Toluidine Blue were counted. Results: The RAW264.7 cell, which induced by RANKL and M-CSF, consistent with morphological characteristies of mature osteoclast, or stained by TRAP method. The Expression of Itgαv、β3 in osteoclast of JGG-group was significantly lower than NS-group(P<0.05), and same as the number and area of bone resorption pits on bone slices . Results: RAW264.7 cell can be induced to mature osteoclast. JGG can effectively inhibit the expression of Itgαv、β3 in osteoclast, and then influence the function of osteoclast.