Selective degradation of an ER stress-induced protein by ER-associated degradation mechanism during stress recovery
Selective degradation of an ER stress-induced protein by ER-associated degradation mechanism during stress recovery
Abstract Unfolded protein response (UPR) is a conserved signaling pathway that is activated by accumulation of misfolded proteins in the endoplasmic reticulum (ER) and stimulates production of ER chaperones to restore ER proteostasis. However, little is known how UPR-induced proteins return to their pre-stress levels upon removal of ER stress. TUNICAMYCIN-INDUCED1 (TIN1) is an Arabidopsis protein that is normally expressed in pollen but is rapidly induced by ER stresses in vegetative tissues. Here we show that the ER stress-induced TIN1 is rapidly degraded in the UPR recovery phase. We found that TIN1 degradation depends on its asparagine-linked glycans and requires both EMS-mutagenized bri1 suppressor 5 (EBS5) and EBS6 for its recruitment to the ER-associated degradation (ERAD) complex. Loss-of-function mutations in Arabidopsis ERAD components greatly stabilize TIN1. Interestingly, two other UPR-induced proteins that are coexpressed with TIN1 remained stable upon removal of ER stress, suggesting that rapid degradation during the stress-recovery phase likely applies to a subset of UPR-induced proteins. Further investigation should uncover the mechanisms by which the ERAD machinery differentially recognizes UPR-induced ER proteins.
Zhang Jianjun、Liu Linchuan、Mao Juan、Lin Liangguang、Wang Pengcheng、Zhang Congcong、Li Jianming、Ma Zhihui、Chen Yongwu、Wang Yi
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agriculture University||Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agriculture University||Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agriculture University||Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural UniversityUniversity of Chinese Academy of Science||Shanghai Center for Plant Stress Biology, The Center of Excellence for Molecular Plant Sciences, Chinese Academy of SciencesUniversity of Chinese Academy of Science||Shanghai Center for Plant Stress Biology, The Center of Excellence for Molecular Plant Sciences, Chinese Academy of SciencesUniversity of Chinese Academy of Science||Shanghai Center for Plant Stress Biology, The Center of Excellence for Molecular Plant Sciences, Chinese Academy of SciencesState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agriculture University||Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University||Department of Molecular, Cellular, and Developmental Biology, University of MichiganState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agriculture University||Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural UniversityUniversity of Chinese Academy of Science||Shanghai Center for Plant Stress Biology, The Center of Excellence for Molecular Plant Sciences, Chinese Academy of SciencesUniversity of Chinese Academy of Science||Shanghai Center for Plant Stress Biology, The Center of Excellence for Molecular Plant Sciences, Chinese Academy of Sciences
分子生物学细胞生物学生物化学
ER-associated degradationUPRTIN1stress recoveryN-glycan
Zhang Jianjun,Liu Linchuan,Mao Juan,Lin Liangguang,Wang Pengcheng,Zhang Congcong,Li Jianming,Ma Zhihui,Chen Yongwu,Wang Yi.Selective degradation of an ER stress-induced protein by ER-associated degradation mechanism during stress recovery[EB/OL].(2025-03-28)[2025-06-27].https://www.biorxiv.org/content/10.1101/2022.06.06.495033.点此复制
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