缩泉丸含药血清对小鼠胚胎干细胞增殖及Nanog基因表达的影响

Objective: To explore effects of Suoquanwan(SQW) drug-containing serum on proliferation activitie and expression of Nanog gene of embryonic stem cell. Methods: Embryonic stem cell line 129 was used as a model. SQW drug-containing serum was added to 129 medium. Flow Cytometer was used to detect nourishing kidney and reducing urination herbals on 129 cell proliferation, Real-Time Quantitative RT-PCR and Flow Cytometer were used to observe the mRNA and protein expression of Nanog gene of embryonic stem cell. Results: SQW drug-containing serum could promote 129 cell proliferation, it also up-regulated Nanog mRNA and protein expression . Conclusion: The method of nourishing kidney and reducing urination could promote 129 cell proliferation, it may relate to raise the key gene Nanog expression.