一种应用于活细胞中检测Hg2+的罗丹明类荧光探针
selective fluorescent sensor based on Rhodamine for imaging mercury in living cells
基于罗丹明类荧光团，合成了1种新型的Hg2+荧光探针R-A。R-A以带有2个羧基的含硫开链冠醚为识别基，对Hg2+表现出较高的选择性和荧光增强，基本不受其他碱金属、碱土金属、过渡金属或重金属离子的干扰。此外，R-A与汞离子有较强的络合能力，不受环境中多种阴离子的影响，并且能够在微摩尔级实现对水相中Hg2+的检测。在较宽的pH范围内（包括生理环境），R-A对Hg2+仍然表现出较大的荧光响应，并且荧光强度保持稳定。R-A的酯化形式，R-E，具有良好的细胞渗透能力，应用于Hela细胞和PC12细胞中显微成像，实现了活细胞内Hg2+的检测。
Based on Rhodamine, a mercury fluorescent sensor R-A was described. The sensor contains two carboxyl-thiol metal-bonding moiety which exhibits highly selective response of fluorescence enhancement toward Hg2+ over other alkali and alkaline earth metal ions, transition and heavy metal ions in HEPES buffer solution. Furthermore, R-A has good coordination ability with Hg2+. Varies anions have no interference on the fluorescence enhancement with R-A, and R-A can detect micromolar level of Hg2+ in aqueous solution. The fluorescence enhancement of R-A towards Hg2+ maintains stable in wide pH span aqueous solution. R-E, the membrane-permeable ethyl ester of R-A, was successfully applied to tracking Hg2+ levels in Hela Cells and PC12 cells using confocal microscopy.
王静云、樊江莉、彭孝军、孙平平、孙世国、郭柯馨
生物科学研究方法、生物科学研究技术生物化学生物物理学
分析化学荧光探针汞离子活细胞
analytical chemistryfluorescent sensorHg2+living cells
王静云,樊江莉,彭孝军,孙平平,孙世国,郭柯馨.一种应用于活细胞中检测Hg2+的罗丹明类荧光探针[EB/OL].(2009-07-23)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/200907-507.点此复制
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