miR-24-Bim 通路调控胰腺癌生长及血管生成的体内研究
he in vivo study of miR-24-Bim Pathway Promotes Tumor growth and Angiogenesis in Pancreatic Carcinoma
目的：miRNA是一类小分子RNA，在肿瘤发展的各个阶段都起着调控作用，许多研究都证实该类小分子在胰腺癌的发生发展中起着重要的作用，本研究拟阐明二者间的相关作用机制。 方法法：选取胰腺癌及癌旁组织，通过慢病毒转染改变miR-24表达的胰腺癌细胞系为研究对象，采用qPCR，Western Blot等方法评估miR-24与Bim之间的表达关系，进一步利用荧光素酶报告系统证实二者之间直接作用关系，通过流式细胞技术，免疫组织化学，细胞增殖检测，血管成环实验等阐明相关生物学效应。 结果果 ：本研究发现细胞凋亡相关蛋白Bim在胰腺癌组织及细胞系中表达明显下降，而Bim上游的miR-24表达明显升高，并证实了二者具有互相调控关系。该通路的相互作用最终加速了肿瘤细胞及血管内皮细胞的生长，促进了血管成环趋势。进一步在体内研究中亦证实了miR-24通过抑制Bin表达能够促进胰腺癌生长及血管生成， 结论：本研究证实了miR-24-Bim的互相调控关系，并阐明了由此产生的生物学功能，提示miR-24 和 Bim可能会成为胰腺癌治疗的靶点。?????
miRNAs are a group of small RNAs that have been reported to play a key role at each stage of tumorigenesis and are believed to have future practical value. We now demonstrate that Bim, which stimulates cell apoptosis, is obviously down-regulated in pancreatic cancer (PaC) tissues and cell lines. And Bim-related miR-24 is significantly up-regulated in Pac. The repressed expression of Bim is proved to be a result of miR-24, thus promoting cell growth of both cancer and vascular cells, and accelerating vascular ring formation. By using mouse tumor model, we clearly showed that miR-24 promotes tumor growth and angiogenesis by suppressing Bim expression in vivo. Therefore, a new pathway comprising miR-24 and Bim can be used in the exploration of drug-target therapy of PaC.?????
巴一、刘锐
肿瘤学基础医学分子生物学
肿瘤学BIMmiR-24胰腺癌肿瘤生长血管生成
OncologyBimmiR-24pancreatic cancertumorigenesisangiogenesis?????
巴一,刘锐.miR-24-Bim 通路调控胰腺癌生长及血管生成的体内研究[EB/OL].(2016-05-30)[2025-08-10].http://www.paper.edu.cn/releasepaper/content/201605-1587.点此复制
评论