Diffusion-weighted magnetic resonance spectroscopy enables cell-specific monitoring of astrocyte reactivity in vivo
Diffusion-weighted magnetic resonance spectroscopy enables cell-specific monitoring of astrocyte reactivity in vivo
Abstract The diffusion of brain intracellular metabolites, as measured using diffusion-weighted magnetic resonance spectroscopy in vivo, is thought to specifically depend on the cellular structure constraining them. However, it has never been established that variations of metabolite diffusion, e.g. as observed in some diseases, could indeed be linked to alterations of cellular morphology. Here we demonstrate, in a mouse model of reactive astrocytes, that advanced diffusion-weighted magnetic resonance spectroscopy acquisition and modeling techniques enable non-invasive detection of reactive astrocyte hypertrophy (increased soma radius, increased fiber radius and length), as inferred from variations of myo-inositol diffusion, and as confirmed by confocal microscopy ex vivo. This establishes that specific alterations of intracellular metabolite diffusion can be measured and related to cell-specific morphological alterations. Furthermore, as reactive astrocytes are a hallmark of many brain pathologies, this work opens exciting perspectives for neuroscience and clinical research.
Hantraye Philippe、Flament Julien、Brouillet Emmanuel、Valette Julien、Hern¨￠ndez-Garz¨?n Edwin、Carrillo-de Sauvage Mar¨aa-Angeles、Bonvento Gilles、Escartin Carole、Palombo Marco、Ligneul Cl¨|mence
Commissariat ¨¤ l?ˉEnergie Atomique et aux Energies Alternatives (CEA)||Centre National de la Recherche Scientifique (CNRS), Universit¨| Paris-Sud, Universit¨| Paris-Saclay, UMR 9199, Neurodegenerative Diseases Laboratory||Institut National de la Sant¨| et de le Recherche M¨|dicale (INSERM)Commissariat ¨¤ l?ˉEnergie Atomique et aux Energies Alternatives (CEA)||Institut National de la Sant¨| et de le Recherche M¨|dicale (INSERM)Commissariat ¨¤ l?ˉEnergie Atomique et aux Energies Alternatives (CEA)||Centre National de la Recherche Scientifique (CNRS), Universit¨| Paris-Sud, Universit¨| Paris-Saclay, UMR 9199, Neurodegenerative Diseases LaboratoryCommissariat ¨¤ l?ˉEnergie Atomique et aux Energies Alternatives (CEA)||Centre National de la Recherche Scientifique (CNRS), Universit¨| Paris-Sud, Universit¨| Paris-Saclay, UMR 9199, Neurodegenerative Diseases LaboratoryCommissariat ¨¤ l?ˉEnergie Atomique et aux Energies Alternatives (CEA)||Centre National de la Recherche Scientifique (CNRS), Universit¨| Paris-Sud, Universit¨| Paris-Saclay, UMR 9199, Neurodegenerative Diseases LaboratoryCommissariat ¨¤ l?ˉEnergie Atomique et aux Energies Alternatives (CEA)||Centre National de la Recherche Scientifique (CNRS), Universit¨| Paris-Sud, Universit¨| Paris-Saclay, UMR 9199, Neurodegenerative Diseases LaboratoryCommissariat ¨¤ l?ˉEnergie Atomique et aux Energies Alternatives (CEA)||Centre National de la Recherche Scientifique (CNRS), Universit¨| Paris-Sud, Universit¨| Paris-Saclay, UMR 9199, Neurodegenerative Diseases LaboratoryCommissariat ¨¤ l?ˉEnergie Atomique et aux Energies Alternatives (CEA)||Centre National de la Recherche Scientifique (CNRS), Universit¨| Paris-Sud, Universit¨| Paris-Saclay, UMR 9199, Neurodegenerative Diseases LaboratoryDepartment of Computer Science and Centre for Medical Image Computing, University College of LondonCommissariat ¨¤ l?ˉEnergie Atomique et aux Energies Alternatives (CEA)||Centre National de la Recherche Scientifique (CNRS), Universit¨| Paris-Sud, Universit¨| Paris-Saclay, UMR 9199, Neurodegenerative Diseases Laboratory
神经病学、精神病学基础医学生物科学研究方法、生物科学研究技术
Intracellular diffusiondiffusion-weightingMRSreactive astrocytesdiffusion modelingbrain microstructuremyo-inositol
Hantraye Philippe,Flament Julien,Brouillet Emmanuel,Valette Julien,Hern¨￠ndez-Garz¨?n Edwin,Carrillo-de Sauvage Mar¨aa-Angeles,Bonvento Gilles,Escartin Carole,Palombo Marco,Ligneul Cl¨|mence.Diffusion-weighted magnetic resonance spectroscopy enables cell-specific monitoring of astrocyte reactivity in vivo[EB/OL].(2025-03-28)[2025-06-10].https://www.biorxiv.org/content/10.1101/350306.点此复制
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