Skp2受肝细胞生长因子影响对人肝癌细胞增殖的作用

Objective To investigate the effect of hepatocyte growth factor （HGF）on the proliferation in human hepatocellualr carcinoma cells, and the mechanism of HGF-induced proliferation inhibition. Methods Human hepatocellualr carcinoma cells HepG2 were treated with different concentrations of HGF for different times, and the proliferation of these cells were examined by colorimetric BrdU cell proliferation enzyme-linked immunosorbent assay. The expression of S-phase kinase associated protein 2(Skp2) was examined using Western blot and RT-PCR. Results HGF inhibited the proliferation of hepatoma cells in a dose and time dependent manner. The expression of Skp2 was significantly suppressed by HGF. Conclusions This study suggested that HGF could inhibit HepG2 cell proliferation, and the down-regulation of Skp2 might account for this suppressed proliferation.