基因工程菌自诱导IL-7蛋白表达的研究

he possibility of using lactose auto-induction to substitute the common inducer IPTG in the fermentation process of the recombinant microorganism in E.coli was deeply investigated. The influences of culture conditions such as lactose concentration, inoculums concentration, DO concentration on the expression of the recombinant protein were analyzed and studied in detail. The result indicated that lactose could induce foreign protein expression and enhance cell growth during the induction period. The IL-7 expression level of auto-induction without antibiotics in medium could got as same the level as that with IPTG as inducer. And the OD600 reached about 171% of the level with IPTG as inducer.