Saliva, a relevant alternative sample for SARS-CoV2 detection
Saliva, a relevant alternative sample for SARS-CoV2 detection
Abstract BackgroundCurrently, COVID-19 diagnosis relies on quantitative reverse-transcriptase polymerase chain reaction (RT-qPCR) from nasopharyngeal swab (NPS) specimens, but NPSs present several limitations. The simplicity, low invasive and possibility of self-collection of saliva imposed this specimen as a relevant alternative for SARS-CoV-2 detection. However, the discrepancy of saliva test results compared to NPSs made of its use controversial. Here, we proposed to assess Salivettes?, as a standardized saliva collection device, and to compare SARS-CoV-2 positivity on paired NPS and saliva specimens. MethodsA total of 303 individuals randomly selected among those investigated for SARS-CoV-2 were enrolled, including 30 (9.9%) patients previously positively tested using NPS (follow-up group), 90 (29.7%) mildly symptomatic and 183 (60.4%) asymptomatic. ResultsThe RT-qPCR revealed a positive rate of 11.6% (n=35) and 17.2% (n=52) for NPSs and saliva samples, respectively. The sensitivity and specificity of saliva samples were 82.9% and 91.4%, respectively, using NPS as reference. The highest proportion of discordant results concerned the follow-up group (33.3%). Although in the symptomatic and asymptomatic groups the agreement exceeded 90.0%, 17 individuals were detected positive only in saliva samples, with consistent medical arguments. ConclusionSaliva collected with Salivette? demonstrated more sensitive for detecting symptomatic and pre-symptomatic infections.
Granjeaud Samuel、Dormoi Jerome、Tissot-Dupont Herv¨|、Benoit Nicolas、Amalvict Remy、Costa Monique Melo、Almeras Lionel、Million Matthieu、Gomez Nicolas、Pradines Bruno
CRCM Integrative Bioinformatics platform, Centre de Recherche en Canc¨|rologie de Marseille, INSERM, U1068, Institut Paoli-Calmettes, CNRS, UMR7258, Aix-Marseille Universit¨| UM 105Unit¨| Parasitologie et Entomologie, D¨|partement Microbiologie et Maladies Infectieuses, Institut de Recherche Biom¨|dicale des Arm¨|es||Aix Marseille Univ, IRD, SSA, AP-HM, VITROME||IHU M¨|diterran¨|e InfectionAix Marseille Univ, IRD, SSA, AP-HM, VITROME||IHU M¨|diterran¨|e InfectionUnit¨| Parasitologie et Entomologie, D¨|partement Microbiologie et Maladies Infectieuses, Institut de Recherche Biom¨|dicale des Arm¨|es||Aix Marseille Univ, IRD, SSA, AP-HM, VITROME||IHU M¨|diterran¨|e Infection||Centre national de r¨|f¨|rence du PaludismeUnit¨| Parasitologie et Entomologie, D¨|partement Microbiologie et Maladies Infectieuses, Institut de Recherche Biom¨|dicale des Arm¨|es||Aix Marseille Univ, IRD, SSA, AP-HM, VITROME||IHU M¨|diterran¨|e Infection||Centre national de r¨|f¨|rence du PaludismeUnit¨| Parasitologie et Entomologie, D¨|partement Microbiologie et Maladies Infectieuses, Institut de Recherche Biom¨|dicale des Arm¨|es||Aix Marseille Univ, IRD, SSA, AP-HM, VITROME||IHU M¨|diterran¨|e InfectionUnit¨| Parasitologie et Entomologie, D¨|partement Microbiologie et Maladies Infectieuses, Institut de Recherche Biom¨|dicale des Arm¨|es||Aix Marseille Univ, IRD, SSA, AP-HM, VITROME||IHU M¨|diterran¨|e InfectionAix Marseille Univ, IRD, SSA, AP-HM, VITROME||IHU M¨|diterran¨|e InfectionUnit¨| Parasitologie et Entomologie, D¨|partement Microbiologie et Maladies Infectieuses, Institut de Recherche Biom¨|dicale des Arm¨|es||Aix Marseille Univ, IRD, SSA, AP-HM, VITROME||IHU M¨|diterran¨|e InfectionUnit¨| Parasitologie et Entomologie, D¨|partement Microbiologie et Maladies Infectieuses, Institut de Recherche Biom¨|dicale des Arm¨|es||Aix Marseille Univ, IRD, SSA, AP-HM, VITROME||IHU M¨|diterran¨|e Infection||Centre national de r¨|f¨|rence du Paludisme
医学研究方法医药卫生理论
salivaCOVID-19 diagnosiscoronavirusSARS-CoV-2
Granjeaud Samuel,Dormoi Jerome,Tissot-Dupont Herv¨|,Benoit Nicolas,Amalvict Remy,Costa Monique Melo,Almeras Lionel,Million Matthieu,Gomez Nicolas,Pradines Bruno.Saliva, a relevant alternative sample for SARS-CoV2 detection[EB/OL].(2025-03-28)[2025-05-23].https://www.medrxiv.org/content/10.1101/2020.11.27.20239608.点此复制
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