PCR污染的防治方法

Polymerase chain reaction (PCR) is a basic molecular biology technique used to amplify specific DNA fragments. In specific experimental procedures, contamination of PCR products or other nucleic acid fragments can lead to false positives, resulting in inaccurate detection results. Aiming at the problem of PCR contamination, various methods have been reported in the existing literature, which are mainly divided into pre-PCR and post-PCR prevention. This paper reviews the prevention methods of PCR contamination, and focuses on how to avoid PCR contamination, so that technicians can save the complicated labor of eliminating contamination after PCR contamination occur.