大分子有机物做碳源对污泥沉降性能的影响研究
Effects of granular substrate on sludge settleability
污泥膨胀的发生与进水水质和运行工况密切相关,为弄清大分子有机物作碳源是否会引发污泥膨胀,以及不同碳源是否会导致不同的优势丝状菌,在3个序批式反应器中,分别采用颗粒性淀粉、溶解性淀粉、葡萄糖作为碳源,研究了碳源类型对污泥沉降性能及微生物种群结构的长期影响。当反应器内底物浓度梯度低导致微生物贮存能力下降时,葡萄糖作碳源的污泥出现了大量丝状菌繁殖,引发了严重污泥膨胀,SVI超过了600 mL/g。而溶解性淀粉作碳源时污泥絮体结构始终松散多孔,SVI值在300 mL/g以内,污泥沉降性能不会严重恶化。颗粒性淀粉做碳源的系统中,由于淀粉的水解速率始终是有机物被利用的限制性因素,所以改变进水方式污泥沉降性变化不大。颗粒性淀粉作碳源时优势丝状菌为0092型和0041型,葡萄糖作碳源时优势丝状菌为S. natans,淀粉作碳源时优势丝状菌为N. limicolaⅡ和M. Parvicella。可见,碳源类型的不同将会导致污泥出现不同的底物代谢特性。
he occurrence of filamentous bulking is associated with specific influent quality and operation schemes. It is still unknown if macromolecular organic substrate might cause filamentous bulking, and if macromolecular organic substrate might result in different dominant filamentous bacteria. In this study, long term effects of macromolecular organic substrate on sludge settleability and the competition between filaments and floc-formers were investigated in order to address these questions. Three lab-scale sequencing batch reactors (SBR) were operated by using particle starch, soluble starch and glucose as the carbon source, respectively. When the storage capability of microorganisms was limited by decreasing of substrate gradient, serious filamentous bulking (SVI>600 mL/g) was caused in the systems with glucose as carbon source. SVI was kept below 300 mL/g in the reactor with soluble starch as carbon source, although a much open, fluffy and porous flocs were formed. Staining reactions and fluorescent in situ hybridization showed that the dominant filaments were type 0092 and type 0041 in particle starch-fed reactor, S. Natans in glucose-fed reactor, N. limicolaⅡ and M. Parvicella in soluble starch-fed reactor. It was demonstrated that various carbon sources would lead to different substrate uptake pathways.
杨雄、王淑莹
环境科学理论环境污染、环境污染防治环境生物学微生物学
淀粉葡萄糖污泥沉降性能污泥膨胀丝状菌
starchglucosesludge settleabilityfilamentous bulkingfilaments
杨雄,王淑莹.大分子有机物做碳源对污泥沉降性能的影响研究[EB/OL].(2013-03-13)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/201303-497.点此复制
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