肝癌血清中岩藻糖结合蛋白的分离与鉴定

urrent biomarkers used clinically still suffer from poor sensitivity and specificity in the diagnosis of hepatocellular carcinoma (HCC). Therefore, new strategy is still needed for discovery of biomarker in HCC. Methods: Here a strategy was performed for the isolation and identification of fucose-binding proteins from the clinical sera (60 normal donors and 60 hepatocellular carcinoma patients) by the fucose-magnetic particle conjugates-based method and LC-MS/MS analysis. Results: 75 proteins for normal donors and 79 proteins for HCC cases were identified, of which 26 were identified in both samples. With the exponentially modified Protein Abundance Index (emPAI) quantification, 8 fucose-binding proteins were estimated down-regulated (emPAI ratio > 2) and 3 MBPs were estimated up-regulated (emPAI ratio < 0.25) in the HCC sera. Conclusions: Fucose-binding proteins were identified as up-regulated in the HCC could be served as potential biomarker and this research also provided new strategy for the discovery of new biomarkers.