枳PtLEAFY 启动子分离与功能鉴定
Isolation and Characterization of Homolog PtLEAFY promoter in Trifoliate Orange (Poncirus trifoliata L. Raf. )
柑橘从播种到开花需经历很长的童期，过长的童期给柑橘的遗传育种带来了很大的麻烦。由于PtLEAFY是控制植株开花的关键基因，通过分离PtLEAFY的上游启动子，对其进行转基因研究发现，PtLEAFY基因在模式植物拟南芥中具有活性，并在植株的真叶，叶脉，下胚轴，花瓣中有表达。这个实验揭示了控制PtLEAFY开花的启动子的活性，并且在一定程度上补充了柑橘开花的理论机理。
bstract The long juvenile phase for 6 to 8 years in woody plants has become a serious obstacle for genetic analysis and breeding practice. In order to understand the flower transition mechanism in woody plants, DNA sequence of a LEAFY homologue in trifoliate orange (Poncirus trifoliata L. Raf.) was isolated and characterized. A chimeric expression construct PtLEAFY::GUS indicated the promoter from woody plant acted its role in Arabidopsis thiliana from 7-day old shoot apical meristem to rosette leaves. These findings concluded that PtLEAFY promoter from woody plants had immediate function in annual plants and may potential provide the specific promoter of shoot apical meristem .
胡春根、张金智、李智敏、梅丽
植物学农业科学研究生物科学研究方法、生物科学研究技术
PtLEAFY启动子转基因拟南芥
LEAFYPromoterransgenic Arabidopsis
胡春根,张金智,李智敏,梅丽.枳PtLEAFY 启动子分离与功能鉴定[EB/OL].(2009-01-19)[2025-08-06].http://www.paper.edu.cn/releasepaper/content/200901-866.点此复制
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