低温诱导黄瓜果实细胞程序性死亡
Evidence of chilling-induced programmed cell death in cucumber fruit
黄瓜（Cucumis sativus L. 品种：浙秀-1）果实在2℃下分别贮藏3、6、9天，然后在20℃下复温2天。果实冷害在复温后明显加重，并伴随着细胞的大量死亡。黄瓜冷藏9天以后再复温2天，乙烯释放量剧烈增加，细胞程序化死亡（PCD）的特征性DNA条带化现象也明显出现。 同时，转移酶介导的三磷酸脱氧鸟苷-生物素刻痕末端标记（TUNEL）检测也发现DNA切痕现象。4,6-二脒基-2-苯基吲哚(DAPI)染色后出现明显的染色质凝聚和细胞核畸形的现象。这些结果表明，黄瓜冷藏后在复温期间冷害加重，大量细胞死亡并表现出明显的PCD特征。乙烯的爆发可能与PCD的出现有着直接联系。
ucumber fruit (Cucumis sativus L. cv. Zhexiu-1) were stored at 2℃ for 3 d, 6 d, 9 d and subsequently reconditioned at 20℃ for 2 d, respectively. Chilling injury (CI) was greatly aggravated upon reconditioning after chilling. Cell death occurred synchronously with CI acceleration. The hallmark of programmed cell death (PCD), DNA laddering, was clearly visualized by genome gel electrophoretic analysis upon reconditioning after chilling for 9 d with ethylene burst. Further evidence of nuclear DNA nicking was confirmed by the in situ TdT-mediated dUTP nick end labelling (TUNEL). In addition, chromatin condensation and nucleus distortion were observed by nuclear staining of DAPI. Results suggested that cell death arisen with the occurrence of chilling injury in cucumber fruit upon reconditioning after cold storage had the features of PCD process which may be mainly attributed to ethylene.
茅林春、陈小红、聂鹏
细胞生物学植物学园艺
采后生物学细胞程序性死亡冷害黄瓜
postharvest biologyprogrammed cell deathchilling injurycucumber
茅林春,陈小红,聂鹏.低温诱导黄瓜果实细胞程序性死亡[EB/OL].(2012-12-27)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201212-941.点此复制
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