The fungal root endophyte Serendipita indica modifies extracellular nucleotides to subvert plant immunity
The fungal root endophyte Serendipita indica modifies extracellular nucleotides to subvert plant immunity
One sentence abstractImmune modulation by metabolites in plant fungus interaction AbstractExtracellular adenosine 5′-triphosphate (eATP) is an essential signaling molecule that mediates different cellular processes through its interaction with membrane-associated receptor proteins in animals and plants. eATP regulates plant growth, development and responses to biotic and abiotic stresses. Its accumulation in the apoplast induces ROS production and cytoplasmic calcium increase mediating a defense response to invading microbes. We demonstrate that perception of eATP is important in plant-fungus interaction and that during colonization by the beneficial root endophyte Serendipita indica accumulation of eATP in the apoplast occurs at early symbiotic stages. We show by liquid chromatography-tandem mass spectrometry, cytological and functional analysis that S. indica subvert eATP host response by secreting SiE5’NT, an enzymatically active ecto-5′nucleotidase capable of hydrolyzing eATP to adenosine. A. thaliana lines producing extracellular SiE5’NT are signi?cantly better colonized and have reduced eATP levels and defense signaling, indicating that SiE5’NT functions as a compatibility factor. Our data show that extracellular bioactive nucleotides play an important role in fungus-root interactions and that fungi can modify plant derived metabolites in the apoplast to modulate host immunity.
Wawra Stephan、Getzke Felix、Schwanke Florian、Zuccaro Alga、Nizam Shadab、Dreyer Ingo、Qiang Xiaoyu、Langen Gregor、Nostadt Robin
University of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne BiocenterUniversity of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne BiocenterUniversity of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne BiocenterMax-Planck-Institute for Terrestrial Microbiology||University of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne BiocenterMax-Planck-Institute for Terrestrial Microbiology||University of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne BiocenterCentro de Bioinform¨￠tica y Simulaci¨?n Molecular (CBSM), Universidad de TalcaMax-Planck-Institute for Terrestrial Microbiology||University of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne BiocenterUniversity of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne BiocenterMax-Planck-Institute for Terrestrial Microbiology
分子生物学植物学微生物学
DORN1extracellular nucleotidesplant immunityLecRK-1.9
Wawra Stephan,Getzke Felix,Schwanke Florian,Zuccaro Alga,Nizam Shadab,Dreyer Ingo,Qiang Xiaoyu,Langen Gregor,Nostadt Robin.The fungal root endophyte Serendipita indica modifies extracellular nucleotides to subvert plant immunity[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/396028.点此复制
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