毛细管电泳法鉴别天麻品种
Identification of Gastrodia elata Bl. by Capillary electrophoresis
发现一种简单、明确的方法,可用于鉴别目前常见的五种天麻。采用超声波水提得待测液;在230nm检测波长,磷酸盐缓冲液稀释5倍后使用,20 KV电压等条件下进行毛细管电泳;所得图谱可见4个较大峰,其中各个品种的1、2、3号峰目测一致,4号峰具有明显的品种特异性,目测即可明显鉴别。
In this paper, a simple, clear method was found. It may be used to identify the five kinds of Gastrodia elata Bl. Menthods: Ultrasonic-assisted extraction of water-solvlable from Gastrodia elata Blume, detection wavelength of 230nm, 5 times diluted phosphate buffer, and 20 KV voltage. Maps in capillary zone electrophoresis are typically obtained in 4 large peaks, various of 1, 2, 3, 4th, and the 4th peak has a significant species specificity.
王德菊、祁岑、赵峰、程立君、黄先敏
中医学药学生物科学研究方法、生物科学研究技术
中药学天麻品种鉴别
hinese Materia MedicaGastrodia elata Bl.Breed discrimination
王德菊,祁岑,赵峰,程立君,黄先敏.毛细管电泳法鉴别天麻品种[EB/OL].(2014-06-12)[2025-07-18].http://www.paper.edu.cn/releasepaper/content/201406-192.点此复制
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