INS-1细胞中CASK对F-actin的作用对胰岛素分泌功能的影响

Objective: Investigate the influence of insulin secretion process by CASK effect on microfilaments F-actin. Methods: 1.Cultured INS-1 cells, certified the impact of CASK on the K+ stimulate insulin secretion （KSIS）function in INS-1 cells . 2.To detect the impact of CASK to the distribution and form of F-actin by using immunofluorescence. 3.To investigate whether CASK change F-actin content by using flow cytometry. Results: 1. Interference the level of CASK was able to reduce KSIS function in INS-1 cells. 3.Both CASK and F-actin might be coordinately regulated insulin secretion in the cell membrane, CASK caused local assembly of F-acitn in INS-1cells. 3. The interference or overexpression of CASK could not change content of F-actin. Conclusion: 1. CASK is associated with KSIS function in INS-1 cells. 2. CASK induce local assembly of F-actin which result in the distribution of F-actin is localized, accordingly，it reduces the barrier role of F-actin obstruct anchor process in insulin secretion ，hence ，it is a possible mechanism that insulin vesicle secretion regulated by CASK. 3. CASK can not affect the content of F-actin.