野菊花组织培养与离体快繁技术研究

In this paper, with the leaves of Chrysanthemum indicum as explants, a set of propagation system in vitro about the tissue culture of induction, differentiation, proliferation, rooting was established. The results showed that the optimal sterilization method for leaves of Chrysanthemum indicum was 70% alcohol 5s + 0.2% NaClO 8 min; optimal early culture medium was MS+ 0.2mg.L-1 6-BA + 0.2mg.L-1 NAA, the temperature is 25℃,the incubation time is 30d and illumination is 12h/d in this method, on which the leave explants could grow vigorously；the optimal proliferation culture medium among all tested media was MS+ 0.2mg.L-1 6-BA + 0.1mg.L-1 NAA. The temperature is 25℃,the incubation time is 30d and illumination is 12h/d；the optimal rooting culture medium among all tested ones was 1/2MS basic medium added 0.2 mgoL-1 NAA. And the temperature is 25℃,the incubation time is 30d and illumination is 12h/d.