Flavin-Tag: A Facile Method for Site-Specific Labeling of Proteins with a Flavin Fluorophore
Flavin-Tag: A Facile Method for Site-Specific Labeling of Proteins with a Flavin Fluorophore
Flavin-tag: we developed a facile method to label a target protein with a flavin. By using a short peptide (Flavin-tag), recognized by a flavinylating enzyme, FMN can be covalently incorporated. Attachment of the flavin chromo- and fluorophore can be performed in vivo and in vitro rendering the Flavin-tag a flexible tool for protein labeling.
Marco Fraaije、Yapei Tong、Jeroen Drenth、Misun Lee
Marco FraaijeYapei TongJeroen DrenthMisun Lee
生物化学生物物理学分子生物学
protein labellingbioconjugating enzymesFMNflavinfluorophorechromophore
Marco Fraaije,Yapei Tong,Jeroen Drenth,Misun Lee.Flavin-Tag: A Facile Method for Site-Specific Labeling of Proteins with a Flavin Fluorophore[EB/OL].(2021-04-23)[2025-08-18].https://chemrxiv.org/engage/chemrxiv/article-details/60c757d4f96a00b1c2288d41.点此复制
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