中国四川地区宫颈癌组织HPV16 E6/E7基因变异研究

ims：To detect the HPV16 E6/E7 variants in Sichuan Province of China and to explore the relationship between the variants and the clinic-pathological parameters of cervical cancer. Methods：Fresh tissue samples from 122 cervical cancer patients and 138 non-malignant controls were analyzed by RT-PCR and bi-directional sequencing. Results：The infection rate of HPV16 in cervical cancer patients in Sichuan province was 70.% (86/122), while in controls the rate was 37.0% (51/138), and the difference was statistically significant (P＜0.001). We got totally 60 sequences of HPV16 E6 in cervical cancer group and 16 in the controls. We found 12 variants with 11 nucleotide mutations. The Asian prototype and the European prototype were the main variant in Sichuan, China. The HPV16R prototype appeared more frequently in the cervical cancer group （9.7%）than in the controls（45.9%）(P＜0.001). Conclusions: HPV16 infection was still the high risk factor for cervical cancer in Sichuan Province of China. The Asian and the European strains were the main variants in this area. The European strain appeared frequently in the non-malignant controls and we deduced that the Asian strain had a higher carcinogenicity than the European strain. Because there were no difference of the variants between the clinic-pathological parameters of cervical cancer, we thought that HPV16 infection was an initial factor in the onset of cervical cancer, and there would be more other factors enrolled in the development of the malignancy.