脐带间充质干细胞对γδ T细胞生物学功能的影响

In this paper, a serial of experiments were designed to determine biological changes in cytokine secretion and cytotoxicity of γδ T cells after co-cultured with UC-MSCs in vitro. MSC were cocultured with PBMCs stimulated by IL-2 and PAM for 12 days at a ratio of 1:5 or 1:80 for 48 hours, and secretion of functional factors such as IFN gamma, TNF, perforin, IL - 10, Granzyme B ofγδ T cells were analyzed by flowcytometry. After the same coculture system for 60 hours, γδ T cells were purified to cocultured with influenza virus H1N1 infected A549s at E:T=10:1，and the dead A549s were analyzed by flowcytometry. The biological function of γδ T cells was inhibited by MSCs. The secretion of IFN gamma was obviously inhibited by MSCs (p < 0.05), and there was no significant change in expression of TNF, Perforin and IL-10 (p>0.05), but the generation of Granzyme B has a rising trend (p < 0.05); but the generation of Granzyme B has a rising trend (p < 0.05). The cytotoxicity of γδ T cells against influenza virus infected A549 was significantly inhibited by MSCs (p < 0.05).