慢性应激对大鼠淋巴细胞DNA损伤机制的研究

Objective] To study DNA damage in peripheral blood lymphocytes under chronic stress. [Methods]By established chronic stress model, and then measured levels of cortisol in blood.Observed the pathomorphism changes in hippocampus with electron microscope. And then the activities of serum superoxide dismutase (SOD) was measured with xanthine oxidase method and the contents of malondiasldehyde (MDA) with thiobarbituric acid in two groups. The degree of DNA damage in peripheral blood lymphocytes was analyzed with comet assay. [Results] Observed the pathomorphism changes in hippocampus with electron microscope. The activities of SOD were lower(56.70±15.65 )U/ml,and contents of MDA in stressful group were higher than the control（9.21±2.75）nmol/ml（P﹤0.05）.The activity of SOD were lower（P﹤0.05）,and contents of MDA were higher（P﹤0.05） in stressful group.The rate of DNA damage was higher in peripheral blood lymphocytes of rats in stressful group than control（P﹤0.05）. It was positive correlation among serum MDA level. [Conclusion] Chronic stress damage hippocampus,Chronic stress may increase contents of MDA and decrease activities of SOD, give rised to some pathomorphism changes in spleen and DNA damage in peripheral blood lymphocytes of rat.