番茄Dof转录因子SlDof1的克隆及表达分析

In this paper,a Dof homologous protein was selected from the NCBI Database by means of program of homologous alignment according to the published Dof protein in Arabidopsis thaliana and Maize and named SlDof1(XP_004233366), and then the specific primers, being used to amplify the cDNA sequences of SlDof1 (XM_004233318)from wild type(WT) tomato(AC++), were designed according to the DNA corresponding sequences. Bioinformatics analysis , expression pattern analysis and analysis of stress and hormone treatment were used to study the Dof gene SlDof1 utilizing the methods of bioinformatics and Real-time PCR. The results of bioinformatics analysis indicated that the full length cDNA of SlDof1 was 1802 bp, whose open reading frame was 1404 bp, encoding 467 amino acid residue. The Real-time PCR (RT-PCR) expression pattern analysis showed that SlDof1 may play important roles in leaves development and fruit ripening in WT tomato. The RT-PCR results of stress and hormone treatment showed that the expression of SlDof1 was induced at different degree in the leaves and root of WT tomato when the methods of mechanical wounding and salt stress were used, and the expression of SlDof1 was affected evidently by ABA, ACC, IAA, GA3 and ZT。These results lay a foundation for further study on the physiological function of SlDof1 in the growth and development and the ascobate acid metabolism of Solanum lycopersicum.