贝那普利对糖尿病大鼠胰腺组织中TGF-β1和Smad3表达的影响

Objective :To investigate the effect of Benazepril on expression of TGF-β1 and Smad3 and RAS system in pancreatic tissue of diabetic rats. Methods :Healthy male Wistar rats were randomly divided into three groups: normal control group, diabetic rats group and Benazepril-treated group. The normal control group fed with normal diet, diabetic rats group and Benazepril - treated group fed with high fat and high sugar diet. After six weeks diabetic rats group and Benazepril - treated group were injected of streptozotocin to induced diabetic rats .Rats were sacrificed after treatment for eight weeks. Glucose,insulin, weight were observed. The sites and level of TGF -β1 and Smad3 protein were examined by immunohistochemistry and were quantitatively analysised by color pathological image analysis system. Radioimmunoassay used to detect pancreatic tissue concentration of angiotensin Ⅱ. Results:Compared to normal control group,Glucose,insulin weight were significantly decreased in diabetic rats group.The level of TGF-β1 and Smad3 in pancreatic tissue were expressed significant increasely.The angiotensinⅡwas increased in pancreatic tissue. Benazepril administration made all changes much better(p<0.05).Conclusion: TGF-β1/ Smad and RAS system signaling is actived in diabetic nephrology. Benazepril treatment can improve the pancreatic fibrosis and islet function.