柑橘毛状根瞬时转化体系的建立

In order to develop a fast and efficient transient transformation system of Poncirus trifoliate, Agrobacterium rhizogenes MSU440 was employed to infect the radicle of P. trifoliate, then hairy roots were induced and transformed roots were fast distinguished by the expression of red fluorescence protein. Our results showed that T-DNA of the vector had been transformed to hairy root successfully, chimeric plants that contained transgenic roots were also obtained in two month, and the transformation efficiency was 29.8%. This study provides a fast and efficient method for function analysis of genes related to citrus root development and symbiosis.