Structure of the eukaryotic cytoplasmic pre-40S ribosomal subunit
Structure of the eukaryotic cytoplasmic pre-40S ribosomal subunit
Abstract Final maturation of eukaryotic ribosomes occurs in the cytoplasm and requires the sequential removal of associated assembly factors and processing of the immature 20S pre-RNA. Using cryo-electron microscopy (cryo-EM), we have determined the structure of a cytoplasmic pre-40S particle poised to initiate final maturation at a resolution of 3.4 ?. The structure reveals the extent of conformational rearrangements of the 3’ major and 3’ minor domains of the ribosomal RNA that take place during maturation, as well as the roles of the assembly factors Enp1, Ltv1, Rio2, Tsr1, and Pno1 in the process. Altogether, we provide a structural framework for the coordination of the final maturation events that drive a pre-40S particle towards the mature form capable of engaging in translation.
Gerhardy Stefan、Weisser Melanie、Leibundgut Marc、Klingauf-Nerurkar Purnima、Panse Vikram Govind、Pe?a Cohue、Ban Nenad、Scaiola Alain、B?hringer Daniel
Institute of Medical Microbiology, University of ZurichInstitute of Molecular Biology and Biophysics, Department of BiologyInstitute of Molecular Biology and Biophysics, Department of BiologyInstitute of Biochemistry, Department of Biology||Institute of Medical Microbiology, University of ZurichInstitute of Medical Microbiology, University of ZurichInstitute of Biochemistry, Department of Biology||Institute of Medical Microbiology, University of ZurichInstitute of Molecular Biology and Biophysics, Department of BiologyInstitute of Molecular Biology and Biophysics, Department of BiologyInstitute of Molecular Biology and Biophysics, Department of Biology
细胞生物学分子生物学生物科学研究方法、生物科学研究技术
pre-40S ribosomecryo-EMribosome assemblyribosomeribosome biogenesis
Gerhardy Stefan,Weisser Melanie,Leibundgut Marc,Klingauf-Nerurkar Purnima,Panse Vikram Govind,Pe?a Cohue,Ban Nenad,Scaiola Alain,B?hringer Daniel.Structure of the eukaryotic cytoplasmic pre-40S ribosomal subunit[EB/OL].(2025-03-28)[2025-04-30].https://www.biorxiv.org/content/10.1101/216713.点此复制
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