结肠癌皮下移植瘤模型方法的优化

Objective: To establish a colon cancer xenograft model through the packet filtering optimum conditions. Methods: According to different cell media and the injection site, the HT29 human colon cancer cell lines were washed with PBS and resuspended in serum-free medium after injection in BALB / C mice armpit and neck skin, build first-generation xenograft model, as a xenograft source, using interpolation block method, and homogenized enzymatic method, a second-generation xenograft model, choose the most preferred method of screening after the establishment of the third generation of subcutaneous xenograft model, by observing tumor growth conditions and metastasis, histopathology, immunohistochemistry, flow cytometry to detect transplanted tumor. Results: The success of established operability, high repeatability colon cancer xenograft model. Observed tumor biopsy showed that the tumor was adenocarcinoma I ~ Ⅱ grade. Immunohistochemistry showed positive expression of CK19 and P53. Flow cytometry analysis of DNA ploidy check aneuploid.Conclusion: In this study, we successfully established a proper screening and colon cancer xenograft model use human colon cancer cell line HT29with BALB/C mice,and provides a good technical platform for the next step in the biological research of colon cancer metastasis model .?????