人类p100蛋白:可增强STAT6基因转录活性的新因子

Objective: To setup a stable cell line which can overexpress human p100 protein, and then study the physiologic functions of p100 in regulation of STAT6-mediated gene transcription. Methods: Using coimmunoprecipitation method to identify protein-protein interactions. STAT6-mediated gene transcriptional activation was detected by luciferase assay. Results: Human p100 protein interacted with both STAT6 and RNA polymerase II, resulting in the enhancement of STAT6-mediated gene transcriptional activation. Conclusion: Human p100 protein acts as a bridging factor between STAT6 and basal transcriptional mechinary.