苯酚降解菌的分离及其降解效率的测定

hree objective strains, named as DP-1、DP-5 and DP-16 respectively, were selected from the samples, which were taken from farm and ponds in the suburbs of Zhengzhou, by methods of enrichment medium culture, so that the strains could live on phenol as sole carbon source. It was showed that DP-5 had the best abilities of degradation in comprison with the other two strains, and when the concentrations of phenol were 500mg/L and 1500 mg/L, the degradation efficiency could be up to 68% and 56% respectively. The content of phenol could be determined by 4-Amino Antipyrine Spectrophotometry, but the results could be affected greatly by the adding order of reagents. The presence of glucose did not promote its degradation efficiency expectedly, on the contrary, because of the priority in the use of glucose, the degradation capacity dropped significantly.