脐带来源的间充质干细胞的分离、传代培养及其分化为成骨和成脂细胞

Objective: Explore the method for isolation and culture of human umbilical cord-derived mesenchymal stem cells(hUC-MSCs) from the umbilical cord, and study the potential for the multiple differentiation of hUC-MSCs. Methods: hUC-MSCs were isolated form human umbilical cord by tissue implant method; hUC-MSCs were induced to the osteogenic and adipogenic differentiation. Results:After 7 days of culture, a few cells migrated out from the placenta tissue and grew in ray-like arrangement, Cells were passaged every 2 to 3 days and grew parallelly; After adipogenic differentiation for 21 days, fat droplets can be stained bright red by oil red O; After osteogenic differentiation for 21 days, calcium nodules can be stained red by Alizarin red. Conclusion:Tissue implant is a good method to obtain hUC-MSCs. hUC-MSCs can be induced to differentiate into osteogenic and adipogenic cells, indicating that hUC-MSCs have the potential of multi- differentiation.