Ccdc38 is required for sperm flagellum biogenesis and male fertility in mouse
Ccdc38 is required for sperm flagellum biogenesis and male fertility in mouse
Abstract Sperm flagellum is essential for male fertility, defects in flagellum biogenesis are associated with male infertility. Deficiency of CCDC42 is associated with malformation of the mouse sperm flagella. Here, we find that the testis-specific expressed protein CCDC38 (coiled-coil domain containing 38) interacts with CCDC42 and localizes on manchette and sperm tail during spermiogenesis. Inactivation of CCDC38 in male mice results in distorted manchette, multiple morphological abnormalities of the flagella (MMAF) of spermatozoa, and eventually male sterility. Furthermore, we find that CCDC38 interacts with intra-flagellar transport protein 88 (IFT88) as well as the outer dense fibrous 2 (ODF2), and its depletion reduces the transportation of ODF2 to flagellum. Altogether, our results uncover the essential role of CCDC38 during sperm flagellum biogenesis, and suggesting the defects of these genes might be associated with male infertility in human being. Summary statementWe demonstrated that CCDC38, localizes on manchette and sperm tail, is crucial for male fertility.
Zhang Ruidan、Liu Chao、Wang Liying、Wu Bingbing、Wang Xiuge、Li Wei、Chen Yinghong、Wei Huafang、Gao Fei、Yuan Li、Xiao Sai
State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences||University of the Chinese Academy of SciencesState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences||Guangzhou Women and Children?ˉs Medical Center, Guangzhou Medical UniversityState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences||Guangzhou Women and Children?ˉs Medical Center, Guangzhou Medical UniversityState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences||University of the Chinese Academy of SciencesState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences||University of the Chinese Academy of SciencesState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences||Guangzhou Women and Children?ˉs Medical Center, Guangzhou Medical UniversityState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences||University of the Chinese Academy of SciencesState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences||Guangzhou Women and Children?ˉs Medical Center, Guangzhou Medical UniversityState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences||University of the Chinese Academy of SciencesSavaid Medical School, University of Chinese Academy of SciencesState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences||University of the Chinese Academy of Sciences
基础医学生理学分子生物学
MMAFCCDC38IFT88ODF2flagellum biogenesis
Zhang Ruidan,Liu Chao,Wang Liying,Wu Bingbing,Wang Xiuge,Li Wei,Chen Yinghong,Wei Huafang,Gao Fei,Yuan Li,Xiao Sai.Ccdc38 is required for sperm flagellum biogenesis and male fertility in mouse[EB/OL].(2025-03-28)[2025-05-15].https://www.biorxiv.org/content/10.1101/2022.01.10.475757.点此复制
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