细胞质酵母双杂交筛选与乙型肝炎病毒X蛋白相互作用的肝细胞蛋白

o screen the hepatocelluar proteins interacting with HBx protein of hepatitis B virus, HBx gene was amplified by means of PCR and cloned into the bait vector pSos. After exclusion of self-activation capacities of HBx and verification of HBx expression in yeast strain cdc25Hα, CytoTrap yeast two hybrid assay was performed using a human liver cDNA library to screen the liver cell proteins interacting with HBx. 30 liver cell proteins interacting with HBX were screened including MYLK、TCTP、EKI1、ECSIT、MRLC2、VCP、USP15、PSMC1、PSMD4、YWHAH、FN1、NACA、MPHOSPH6、GSTO1、TOMM70A、ORM2、ITIH3、SF3A3、PREPL、PTPRA、SMARCC1、BRWD2、CP、ETNK1、PPM1A、LSM8、AMACR、AFM、IQWD1、RBX1. HBx may effect multifarious function of hepatocytes such as cell signal pathways, proteins degradation, tumor invasion, metastasis and apoptosis.