Heterogeneity of murine periosteum osteochondroprogenitors involved in fracture healing
Heterogeneity of murine periosteum osteochondroprogenitors involved in fracture healing
Abstract The periosteum is the major source of cells involved in fracture healing. We sought to characterize differences in progenitor cell populations between periosteum and other bone compartments, and identify periosteal cells involved in fracture healing. The periosteum is highly enriched for progenitor cells, including Sca1+ cells, CFU-F and label-retaining cells. Lineage tracing with αSMACreER identifies periosteal cells that contribute to >80% of osteoblasts and ~40% of chondrocytes following fracture. A subset of αSMA+ cells are quiescent long-term injury-responsive progenitors. Ablation of αSMA+ cells impairs fracture callus formation. In addition, committed osteoblast-lineage cells contributed around 10% of osteoblasts, but no chondrocytes in fracture calluses. Most periosteal progenitors, particularly those that form osteoblasts, can be targeted by αSMACreER. We have demonstrated that the periosteum is highly enriched for skeletal stem and progenitor cells and there is heterogeneity in the populations of cells that contribute to mature lineages during periosteal fracture healing.
Novak Sanja、Funnell Jessica L.、Matthews Brya G、Kalajzic Ivo、Grcevic Danka、Buckels Emma J.、Cao Ye、Sbrana Francesca V
Department of Reconstructive Sciences, UConn Health UConn HealthDepartment of Reconstructive Sciences, UConn Health UConn HealthDepartment of Molecular Medicine and Pathology, University of Auckland||Department of Reconstructive Sciences, UConn Health UConn HealthDepartment of Reconstructive Sciences, UConn Health UConn HealthDepartment of Physiology and Immunology, University of ZagrebDepartment of Molecular Medicine and Pathology, University of AucklandDepartment of Molecular Medicine and Pathology, University of AucklandDepartment of Reconstructive Sciences, UConn Health UConn Health
基础医学细胞生物学生理学
Periosteumskeletal stem cellprogenitoralpha smooth muscle actinosteoblastfracture
Novak Sanja,Funnell Jessica L.,Matthews Brya G,Kalajzic Ivo,Grcevic Danka,Buckels Emma J.,Cao Ye,Sbrana Francesca V.Heterogeneity of murine periosteum osteochondroprogenitors involved in fracture healing[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2020.06.24.169003.点此复制
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