Genome-wide identification of fitness determinants in the Xanthomonas campestris bacterial pathogen during early stages of plant infection
Genome-wide identification of fitness determinants in the Xanthomonas campestris bacterial pathogen during early stages of plant infection
Abstract Plant diseases are an important threat to food production. While major pathogenicity determinants required for disease have been extensively studied, less is known on how pathogens thrive during host colonization especially at early infection stages. Here, we used randomly barcoded-transposon insertion site sequencing (RB-TnSeq) to perform a genome-wide screen and identify key bacterial fitness determinants of the vascular pathogen Xanthomonas campestris pv. campestris (Xcc) during infection of the cauliflower host plant (Brassica oleracea). This high-throughput analysis was conducted in hydathodes, the natural entry site of Xcc, in xylem sap and in synthetic media. Xcc did not face a strong bottleneck during hydathode infection. 183 genes important for fitness were identified in plant-associated environments with functional enrichment in genes involved in metabolism when only few genes known to be involved in virulence were found to be affected. The biological relevance of 13 genes was independently confirmed by phenotyping single mutants. Notably, we show that the XC_3388, a protein with no known function (DUF1631), plays a key role in the adaptation and virulence of Xcc possibly through c-di-GMP-mediated regulation. This study thus revealed yet unsuspected social behaviors adopted by Xcc individuals when confined inside hydathodes at early infection stages.
Boulanger Alice、Baudin Ma?l、Quiroz-Monnens Thomas、Carr¨¨re S¨|bastien、Torralba Babil、Lewis Jennifer D.、Lauber Emmanuelle、Jardinaud Marie-Fran?oise、No?l Laurent D.、Ray Jayashree、Arlat Matthieu、Luneau Julien S.、Deutschbauer Adam M.、Bouchez Olivier、Fran?ois Jonas、Gris Carine
LIPME, Universit¨| de Toulouse, INRAE, CNRS, Universit¨| Paul SabatierPlant Gene Expression Center, USDA||Department of Plant and Microbial Biology, University of CaliforniaLIPME, Universit¨| de Toulouse, INRAE, CNRS, Universit¨| Paul SabatierLIPME, Universit¨| de Toulouse, INRAE, CNRS, Universit¨| Paul SabatierLIPME, Universit¨| de Toulouse, INRAE, CNRS, Universit¨| Paul SabatierPlant Gene Expression Center, USDA||Department of Plant and Microbial Biology, University of CaliforniaLIPME, Universit¨| de Toulouse, INRAE, CNRS, Universit¨| Paul SabatierLIPME, Universit¨| de Toulouse, INRAE, CNRS, Universit¨| Paul SabatierLIPME, Universit¨| de Toulouse, INRAE, CNRS, Universit¨| Paul SabatierEnvironmental Genomics and Systems Biology Division, Lawrence Berkeley National LaboratoryLIPME, Universit¨| de Toulouse, INRAE, CNRS, Universit¨| Paul SabatierLIPME, Universit¨| de Toulouse, INRAE, CNRS, Universit¨| Paul SabatierDepartment of Plant and Microbial Biology, University of California||Environmental Genomics and Systems Biology Division, Lawrence Berkeley National LaboratoryGenotoul Genome & Transcriptome (GeT-PlaGe), INRAELIPME, Universit¨| de Toulouse, INRAE, CNRS, Universit¨| Paul SabatierLIPME, Universit¨| de Toulouse, INRAE, CNRS, Universit¨| Paul Sabatier
微生物学分子生物学植物学
RB-TnSeqXanthomonasFitnessBrassica oleraceaHydathodesXylem
Boulanger Alice,Baudin Ma?l,Quiroz-Monnens Thomas,Carr¨¨re S¨|bastien,Torralba Babil,Lewis Jennifer D.,Lauber Emmanuelle,Jardinaud Marie-Fran?oise,No?l Laurent D.,Ray Jayashree,Arlat Matthieu,Luneau Julien S.,Deutschbauer Adam M.,Bouchez Olivier,Fran?ois Jonas,Gris Carine.Genome-wide identification of fitness determinants in the Xanthomonas campestris bacterial pathogen during early stages of plant infection[EB/OL].(2025-03-28)[2025-05-11].https://www.biorxiv.org/content/10.1101/2022.02.07.479439.点此复制
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