anisomycin对小鼠结肠癌CT26的体内外作用

aim: to observe preliminary effect of anisomycin on CT26 cells in vitro and in vivo. Methods: MTT was employed to detect the proliferation of CT26 cells treated with different time and different concentrations of anisomycin. Flow cytometry was used to determine how anisomycin affects CT26 cell cycle and apoptosis. In vivo therapy was taken out to the tumor-bearing mice by intratumor injection of anisomycin or doxorubicin. Results: Anisomycin inhibited significantly CT26 cell proliferation. The CT26 cell cycle entry was mainly blocked at S-phase with the enhancement of the Sub-G0/G1-phase proportion and the CT26 cell apoptosis was increased by anisomycin. The tumor volume was reduced in the CT26-bearing mice treated with anisomycin. Conclusion: anisomycin inhibits growth of CT26 cells both in vitro and in vivo.