流场内不同压力环境对内皮细胞流变学特征的影响

The circulation system is a closed pressure-loaded system, the pressure in circulation flow field would change followed the nature or pathological geometry change of the local blood vessel, and the pressure shift induced by the vascular geometry change would lead to a series physiological and pathological changes in the endothelial cells(ECs). The experiment is designed to elucidate the effects of different pressure shift on F-actin alignment and expression in cultured endothelial cells in vitro, and to investigate the relationship between the altered pressure shift and the expression intensity of Vascular adhesion molecule (VCAM) and IntegrinαVβ3. Non-activated cultured ECs and single shear stress loaded ECs as control group were set, the double-immuno-fluoro-cytochemistry, laser confocal scanning microscopy and image analysis system were used to observe the expression of VCAM、IntegrinαVβ3 and F-actin in endothelial cells which were exposed to levels of pressure shift in a improved parallel plate flow chamber. When exposed to different decreased pressure shift, the expression intensity of VCAM、IntegrinαVβ3 and F-actin showed regular changes. The decreased pressure shift resulted in changes in cell alignment and cytoskeleton F-actin, and also affected ECs adhesion function and transmembrane mechanotransduction function which were represented by VCAM and IntegrinαVβ3 respectively.