番茄根霉果腐病原菌拮抗菌的筛选及拮抗机理研究
Screening of antagonistic strain against hizopus nigricans Ehrenb and study on mechanism of antagonism
为防治番茄根霉果腐病，筛选出一株对该病原菌有高效拮抗作用的微生物并研究其拮抗机理。对土壤中的微生物进行番茄果实体内和体外的抗性筛选，得到一株对番茄根霉果腐病原菌有高效拮抗作用的微生物⑤12.3。经过初步鉴定，⑤12.3为一株假单胞菌。番茄果实果腐病菌接种试验表明拮抗菌⑤12.3处理可100%抑制果实发病，且对叶霉病也有抑制作用。菌株次生代谢产物的验证表明⑤12.3自身可产生苯丙氨酸解氨酶和几丁质酶。用⑤12.3处理后番茄果实抗性相关酶POD、PPO的活性分别提高到原来的2.9和2.2倍。由此可见，假单胞菌⑤12.3对番茄根霉果腐病原菌有高效的拮抗作用，并且它的拮抗作用是由多种机制共同调节的。
In order to control hizopus nigricans Ehrenb，one strain antagonistic (⑤12.3) to hizopus nigricans Ehrenb which obtained from soil was obtained，mechanism of antagonism was studied．After the in vivo and in vitro experiment，one strain antagonistic (⑤12.3) to hizopus nigricans Ehrenb which obtained from soil was obtained. Based on the phenotypic characteristic and physiochemical experiment，⑤12.3 was identified as Pseudomona ssp primarily．Tomato inoculated with ⑤12.3 can inhibit hizopus nigricans Ehrenb completely as well as Fulviafulva (Cooke) Cifer．Based on the identification of microorganism metabolism production，⑤12.3 could produce antibiotic substances like PAL and chitinous by itself．After inoculated with ⑤12.3，tomato’s POD activity and PPO activity increase to 2.9 times and 2.2 times．The results showed that ⑤12.3 is a effective antagonistic bacteria．It’s antibacterial mechanism was multiplicate．
生吉萍、王菲、申琳
微生物学植物学生物科学研究方法、生物科学研究技术
生物防治，拮抗细菌，假单胞菌，拮抗机理，番茄根霉果腐病
biological control，antagonistic bacteria，Pseudomona ssp，mechanism of antagonism，hizopus nigricans Ehrenb．
生吉萍,王菲,申琳.番茄根霉果腐病原菌拮抗菌的筛选及拮抗机理研究[EB/OL].(2008-03-20)[2025-08-03].http://www.paper.edu.cn/releasepaper/content/200803-590.点此复制
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