Gibberellin and the miRNA156-targeted SlSBPs synergistically regulate tomato floral meristem activity and fruit patterning
Gibberellin and the miRNA156-targeted SlSBPs synergistically regulate tomato floral meristem activity and fruit patterning
ABSTRACT Many developmental processes associated with fruit development take place at the floral meristem (FM). Age-regulated microRNA156 (miR156) and gibberellins (GA) interact to control flowering time, but their interplay in subsequent stages of reproductive development is poorly understood. Here, we show that GA and miR156 function in tomato FM and fruit patterning. High GA responses or overexpression of miR156 (156OE), which leads to low levels of miR156-targeted SQUAMOSA PROMOTER BINDING PROTEIN– LIKE (SPL/SBP), resulted in enlarged FMs, defects in FM determinacy and fruits with increased locule number. Conversely, low GA responses reduced fruit indeterminacy and locule number, and overexpression of a miR156-resistant SlSBP15 allele (rSBP15) reduced cell number and size in the FM, as well as locule number. GA responses were partially required for the fruit defects observed in 156OE and rSBP15 plants. Transcriptome analysis and genetic interactions revealed shared and divergent functions of miR156-targeted SlSBPs, PROCERA/DELLA and the classical WUSCHEL/CLAVATA pathway, which has been previously associated with meristem size and determinacy. Our findings reveal that the miR156/SlSBP/GA regulatory module is deployed differently depending on developmental stage and create novel opportunities to genetically fine-tune aspects of fruit development that have been important for tomato domestication.
Silva Geraldo F.F.、Vicente Mateus H.、Barrera-Rojas Carlos H.、Silva Eder M.、Peres Lazaro E.P.、Ambrosano Guilherme B.、Margarido Gabriel R. A.、Sablowski Robert、Nogueira Fabio T.S.、Carvalho Airton Jr、Ferigolo Leticia F.、Correa Joao P. O.
Laboratory of Molecular Genetics of Plant Development University of Sao PauloLaboratory of Molecular Genetics of Plant Development University of Sao PauloLaboratory of Molecular Genetics of Plant Development University of Sao PauloLaboratory of Molecular Genetics of Plant Development University of Sao PauloLaboratory of Hormonal Control of Plant Development University of Sao Paulo (USP) 13418-900 PiracicabaDepartment of Genetics, University of S?o Paulo Escola Superior de Agricultura ?°Luiz de Queiroz?± (ESALQ), University of Sao PauloDepartment of Genetics, University of S?o Paulo Escola Superior de Agricultura ?°Luiz de Queiroz?± (ESALQ), University of Sao PauloCell and Developmental Biology Department, John Innes Centre, Norwich Research ParkLaboratory of Molecular Genetics of Plant Development University of Sao PauloLaboratory of Molecular Genetics of Plant Development University of Sao PauloLaboratory of Molecular Genetics of Plant Development University of Sao PauloLaboratory of Molecular Genetics of Plant Development University of Sao Paulo
分子生物学植物学遗传学
miR156PROCERA/DELLAfloral meristemfruit patterninggibberellinSlSBP15
Silva Geraldo F.F.,Vicente Mateus H.,Barrera-Rojas Carlos H.,Silva Eder M.,Peres Lazaro E.P.,Ambrosano Guilherme B.,Margarido Gabriel R. A.,Sablowski Robert,Nogueira Fabio T.S.,Carvalho Airton Jr,Ferigolo Leticia F.,Correa Joao P. O..Gibberellin and the miRNA156-targeted SlSBPs synergistically regulate tomato floral meristem activity and fruit patterning[EB/OL].(2025-03-28)[2025-06-15].https://www.biorxiv.org/content/10.1101/2023.05.08.539891.点此复制
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